. . "2554"^^ . . "Elektroporace je metoda genov\u00E9ho in\u017Een\u00FDrstv\u00ED, v r\u00E1mci n\u00ED\u017E je kr\u00E1tk\u00FDm intenzivn\u00EDm elektrick\u00FDm pulsem dosa\u017Eeno vzniku p\u00F3r\u016F v membr\u00E1n\u011B bun\u011Bk. Tyto p\u00F3ry umo\u017E\u0148uj\u00ED vstup cizorod\u00FDch element\u016F, zejm\u00E9na DNA \u010Di r\u016Fzn\u00FDch drog, dovnit\u0159 bun\u011Bk. P\u00F3ry se velmi rychle zacel\u00ED a bu\u0148ky tedy mohou proceduru p\u0159e\u017E\u00EDt. Tento typ genetick\u00E9 transformace se pou\u017E\u00EDv\u00E1 v laborato\u0159i k vn\u00E1\u0161en\u00ED DNA do bakteri\u00E1ln\u00EDch, ale i rostlinn\u00FDch \u010Di \u017Eivo\u010Di\u0161n\u00FDch bun\u011Bk.V p\u0159\u00EDpad\u011B bakteri\u00ED E. coli je protokol \u010Dasov\u011B velmi nen\u00E1ro\u010Dn\u00FD a rychl\u00FD."@cs . . . "Elektroporace je metoda genov\u00E9ho in\u017Een\u00FDrstv\u00ED, v r\u00E1mci n\u00ED\u017E je kr\u00E1tk\u00FDm intenzivn\u00EDm elektrick\u00FDm pulsem dosa\u017Eeno vzniku p\u00F3r\u016F v membr\u00E1n\u011B bun\u011Bk. Tyto p\u00F3ry umo\u017E\u0148uj\u00ED vstup cizorod\u00FDch element\u016F, zejm\u00E9na DNA \u010Di r\u016Fzn\u00FDch drog, dovnit\u0159 bun\u011Bk. P\u00F3ry se velmi rychle zacel\u00ED a bu\u0148ky tedy mohou proceduru p\u0159e\u017E\u00EDt. Tento typ genetick\u00E9 transformace se pou\u017E\u00EDv\u00E1 v laborato\u0159i k vn\u00E1\u0161en\u00ED DNA do bakteri\u00E1ln\u00EDch, ale i rostlinn\u00FDch \u010Di \u017Eivo\u010Di\u0161n\u00FDch bun\u011Bk.V p\u0159\u00EDpad\u011B bakteri\u00ED E. coli je protokol \u010Dasov\u011B velmi nen\u00E1ro\u010Dn\u00FD a rychl\u00FD. Bakterie se opl\u00E1chnou a rozpust\u00ED v roztoku s glycerolem a n\u00EDzkou iontovou silou tak, aby vytvo\u0159ili hustou suspenzi. V\u0161e se dr\u017E\u00ED na ledu, elektroporace nejl\u00E9pe prob\u00EDh\u00E1 p\u0159i 0\u20134 \u00B0C. Po p\u0159id\u00E1n\u00ED z\u00E1jmov\u00E9 DNA do t\u00E9to sm\u011Bsi (nap\u0159. n\u011Bjak\u00E9ho vektoru) se elektropor\u00E1torem aplikuje kr\u00E1tk\u00FD elektrick\u00FD puls (o intenzit\u011B 12.5-15 kV cm-1). U rostlin se postupuje relativn\u011B podobn\u011B, je v\u0161ak nejprve vhodn\u00E9 odstranit jejich bun\u011B\u010Dnou st\u011Bnu a elektroporovat tzv. protoplasty."@cs . . . . "18"^^ . . . . . . . . . . "elektroporac\u00ED"@cs . . . . . "15734476"^^ . . . "Elektroporace"@cs . . "1028750"^^ .